The largest of the gallery's three London sites, providing more than 5440 m2 (58,000 sq ft) of interior space on a site of 1.7 acres (74,300 sq ft). The building dates from the 1970s and was primarily used as a warehouse before the current refurbishment.

White Cube Bermondsey was designed by Casper Mueller Kneer Architects who are based in London and Berlin. The building includes three principal exhibition spaces, substantial warehousing, private viewing rooms, an auditorium and a bookshop. The 'South Galleries' provide the principal display area for White Cube's expanding programme of significant exhibitions. Three smaller galleries, collectively known as the 'North Galleries', feature an innovative new programme of exhibitions. In addition, at the centre of the building, there is a gallery of 81m2 entitled ‘9 x 9 x 9’. An auditorium will allow White Cube to present an education programme, artists’ films and lectures.

White Cube Mason’s Yard opened in September 2006. It is located off Duke Street, St. James's, home of the original White Cube gallery, on a site that was previously an electricity sub-station. Also designed by MRJ Rundell & Associates it is the first free-standing building to be built in the St James's area for more than 30 years. The building houses a main, basement floor gallery which is a naturally lit, double-height space with a second gallery on street level providing 5000 ft² of exhibition space. White Cube Mason’s Yard continues with an international, high profile programme of exhibitions and was launched with an inaugural exhibition by Gabriel Orozco.

In April 2000, White Cube Hoxton Square was set up as a second, larger gallery space in London’s East End. Housed in a 1920s light industrial building, and designed by architects MRJ Rundell and Associates, White Cube Hoxton Square has 2000 square feet of uninterrupted exhibition space.

White Cube Hong Kong, located at 50 Connaught Road, in the heart of Hong Kong’s Central district. lt provides over 550 m² (6000 sqft) of interior space over two floors, including a gallery height of over 4.5 meters. Designed by the London based architects Maybank and Matthews Architects, this is the first of White Cube’s galleries to be located outside of the UK.

'Inside the White Cube' is a programme of exhibitions profiling work by artists who have not previously shown at the gallery. The series provides a stimulating platform for exploring new developments in international contemporary art, across a range of practices and media. Based at White Cube Bermondsey, the exhibitions are adaptable in content and, on occasion, migratory in location: from the north galleries, south galleries and auditorium to courtyard and external sites. 

White Cube was set up by Jay Jopling in 1993 as a project room for contemporary art. Although it was one of the smallest exhibition spaces in Europe, it was arguably one of most influential commercial galleries of the past decade. Situated on the second floor of 44 Duke Street, St James’s, one of London’s most traditional art dealing streets, White Cube Duke Street was, literally, a simple white cube, a room within a room, designed by the architect Claudio Silvestrin.

The central concern when establishing the programme was to create an intimate space in which an artist could present a single important work of art or a coherent body of work within a focused environment, an idea that in some way, stemmed from the memorable experience of Walter de Maria’s ‘Earth Room’ in New York. The programme was singular among commercial galleries in that an artist was invited to exhibit only once. Since its inception, the gallery mounted exhibitions of work by many leading international and British artists including Franz Ackermann, Miroslaw Balka, Chuck Close, Tracey Emin, Katharina Fritsch, Mona Hatoum, Damien Hirst, Gary Hume, Ellsworth Kelly, Julie Mehretu, Doris Salcedo, Hiroshi Sugimoto, Luc Tuymans and Jeff Wall. White Cube, Duke Street closed in 2002.